FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk265.3410000000f20f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk265.3410000000f20f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10789713
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3286863.046290480571633TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT399550.042.57717512
CACGTCT404050.042.06400314
GATCGGA410350.041.9495051
CTCGTAT385200.041.920442
ACACGTC405800.041.89369613
TCTCGTA371350.041.68441841
GTATGCC399250.041.67971845
TCGTATG399050.041.61564643
TCGGAAG414250.041.6152083
CGTCTGA409500.041.4712116
AGCACAC412350.041.3810110
AGAGCAC414150.041.3695688
GAGCACA414850.041.2672239
AGATCTC372150.041.2139338
ACGTCTG413000.041.21237615
GTCTGAA412650.041.1873517
TCTGAAC411400.041.1867118
CAGTCAC406200.041.1489927
AGTCACG406800.041.07723628
GCACACG415550.041.05151711
Produced by FastQC (version 0.11.2)