FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk264.3410000000f1e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk264.3410000000f1e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4200606
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC947902.25657916976741TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT117650.042.41694312
ACACTTG115050.042.1630432
AGTCACA116150.042.03492728
CACACTT116150.041.86058831
TCACACT116500.041.7541430
CACTTGA116000.041.7013633
CTCGTAT113850.041.6390942
ACACGTC120100.041.60785713
TCGTATG117200.041.5239543
CACGTCT119850.041.50691614
TCGGAAG122400.041.3466033
ACTTGAA116700.041.33554534
GTCACAC118100.041.18845729
TCTGAAC121250.041.17611318
GATCGGA123250.041.092431
GAGCACA122850.040.9511769
AGAGCAC122800.040.931218
GTCTGAA122050.040.9246517
ATCGGAA123850.040.872262
TCTCGTA112500.040.818841
Produced by FastQC (version 0.11.2)