FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l02n01 mk263.3410000000f1ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l02n01 mk263.3410000000f1ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11718525
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1652361.410040939452704TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT208850.041.3465612
GTCTGAA212750.040.53574417
CGTCTGA212450.040.4976716
AGTCACC206000.040.42227628
CACGTCT213400.040.3384714
CTCGTAT204450.040.1564842
ACGTCTG215200.040.10561815
GTATGCC208850.040.07569545
CGTATGC209100.040.01667844
GCACACG219400.039.38914511
CAGTCAC214350.039.3724527
TCGTATG212200.039.3578643
AGCACAC220400.039.2308510
TCTGAAC220450.039.0586518
ACACGTC222300.038.7943313
CTGAACT222150.038.7293719
TCTCGTA206750.038.36034441
GATCGGA229300.037.8546031
CCAGTCA224800.037.7023326
AGAGCAC230150.037.6861958
Produced by FastQC (version 0.11.2)