FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 44r.3420000000f0e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 44r.3420000000f0e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6888533
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAC7950.026.0455151
CGGGGCG2300.025.4423791
CGGGGTG6100.023.2446481
CGGGGAG7450.022.9598771
CGGGGAT12150.022.599341
CGGGGGG2850.022.1118661
CGGGGTT11200.020.6981451
CGGGGGT6250.020.526131
CGGGGGA5850.020.0059761
GGGGACA15400.018.1196632
GGGGGAT17250.017.744431
GAGGGAT17250.016.700641
GGGGTCA11350.016.654542
GGGGGCG8600.016.4874821
GCGGGTG9300.016.4565281
TGGGGTT25600.016.4404581
GGCGGGC4550.015.8289041
GGCGGGT8150.015.7408981
GGGGACC8300.015.7253052
GGCGGTT10900.015.692761
Produced by FastQC (version 0.11.2)