FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 44mh.3420000000f184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 44mh.3420000000f184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13839976
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAC11400.024.878141
GGGGGAT51000.022.376391
CGGGGAG17700.021.7457851
CGGGGAT22700.021.5172161
GGGGGAC28850.020.9093821
CGGGGGG7400.020.6837581
CGGGGTG14500.019.5593661
GAGGGAT47150.019.2864321
CGGGGCG5950.018.5366551
GGGGGAA82000.018.4187811
CGGGGTT18250.018.3770451
GGGGACC17800.018.0791172
CGGGGGT11450.017.6925011
CGGGGGA12400.016.8815961
GGGGGTC20300.016.8538761
GAGGGGA38850.016.8019311
GGGGTTA42650.016.6735942
CTCGGTG18950.016.50354844
GAGGGTC25700.016.0276641
GCACACG10400.016.00788518