FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 43r.3420000000f0c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 43r.3420000000f0c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089479
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAC10700.024.402111
CGGGGAG11100.023.3199711
CGGGGGG4800.021.102041
CGGGGGT8200.020.3128571
CGGGGAT19600.019.4081341
GGGGGCG13000.019.3922331
CGGGGCG4150.017.8985961
GGGGTTA30950.017.5242312
CGGGGTG8950.017.3531861
CGGGGTT16300.016.9851991
TGGGGTT46750.015.9848881
GCGGGTG14200.015.6927831
GCGGGAC7500.015.3060121
CGGGGGA8700.015.2646171
GGGGACA23500.015.0353782
GGGGGAT24100.014.9436291
GGGCGTC10000.014.8558361
GAGGGAT27600.014.7612321
GGCGGTG23550.014.4324221
GGCGGGT12050.014.3832441
Produced by FastQC (version 0.11.2)