FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 43mh.3420000000f16a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 43mh.3420000000f16a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10650866
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCG4900.026.6428621
CGGGGAT19850.026.0805231
GCACACG11550.025.12699118
CGGGGAG13750.025.0459611
CGGGGGG5600.024.9202651
CGGGGTG11550.024.7497441
CGGGGAC9650.024.72451
GGGGGAT38150.024.4851361
TGCGCGG14050.023.6983516
GCGCGGT13900.023.63038417
GGGGGAC21950.022.3548071
CGCGGTC15000.021.74750918
CGGGGGT10800.021.466561
GTGCGCG15300.021.32188815
GAGGGAT30050.020.6734811
GGGGGCG15150.020.6514781
CGGGGTT16000.019.9764061
GGGGGAA59850.019.5187591
GGGGACC13000.019.3887672
CGGGGGA9900.019.3255861
Produced by FastQC (version 0.11.2)