FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 42sr.3420000000f0af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 42sr.3420000000f0af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9925641
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG9150.023.8613221
CGGGGAC11000.022.9176041
CGGGGCG4500.022.5083641
CGGGGAG12250.021.6815261
CGGGGGG4650.021.2982351
GCGGGTG13200.018.5864521
GGGGGCG10600.018.2615031
GGGGACC12700.018.0743032
CGGGGAT17650.017.8536591
CGGGGTT14050.017.6222061
GGGGGAC18650.017.379111
GGGGGAT22750.017.2151871
GGCGGTG21400.017.144221
TGGGGTT35600.017.0077251
GGGGTTA24150.016.680192
GAGGGAT26400.016.284461
CGGGGGA7650.016.1824841
GGCGGAA25800.016.1397171
GGGCGAG11350.015.8649251
TGGGGTA32950.015.71145251
Produced by FastQC (version 0.11.2)