FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 42r.3420000000f10d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 42r.3420000000f10d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13404520
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA16250.026.16980441
GGTCGCC17950.024.69413438
GTCGCCG19500.023.07742739
CGGGGAG17250.022.8321251
CGGGGAC12250.022.0466461
TCGCCGT20250.021.22268540
CGGGGAT23500.019.441311
GGGGGAT37500.019.325111
CGGGGTG13000.018.697251
GCGCCCC12750.018.52940431
GCCGTAT24150.018.4475442
CCGTATC24500.017.72461343
CGGGGTT18800.017.717451
CGGGGGA13800.017.6133541
CGGGGGG6550.017.5237161
GGGGACC15900.016.9837232
GGGGGAA69400.016.6038131
TGGGGAC29200.016.2628611
TGCGCCC14750.016.16918230
GGGGGAC23950.016.162941
Produced by FastQC (version 0.11.2)