FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 42mh.3420000000f141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 42mh.3420000000f141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7711193
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAC7800.026.2602371
CGGGGAT14350.023.685191
GGGGGAT26450.021.9556271
CGGGGTG7450.021.1491811
CGGGGTT11800.020.4104981
GGGGGAC15450.020.39631
GGGGACC9750.020.3131052
CGGGGAG10300.020.1049231
GGGGGCG10700.019.5637021
CGGGGGG4200.019.2932341
GCACACG7800.018.74745818
GGGGGAA44200.018.180161
GAGGGAT24350.017.8406281
GGCGGAT15500.017.5713651
CGGGGCG3750.017.4067841
ACGCGTA9750.016.8450715
GGGGGCC10300.016.8269461
ATACGCG10050.016.79116213
TACGCGT9700.016.70114714
CGGGGGT7450.016.6172141
Produced by FastQC (version 0.11.2)