FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 41r.3420000000f085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 41r.3420000000f085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12052003
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAG12750.023.8321551
CGGGGAC12600.023.5799621
CGGGGGG5600.019.2926961
CGGGGAT21500.018.1112061
CGGGGTG10850.018.0480061
GGGGACC12750.018.0036012
CGGGGGT9500.017.7695871
CGGGGCG3650.017.2665211
GAGGGAT35600.016.9443341
GGGGAAC32000.016.808052
GCGGGTG17950.016.6773431
GGGGGAC18550.016.6232641
CGGGGTT19550.016.578891
GGGGGAT29550.016.3003161
TGGGGTT51900.015.9595331
TGGGGTA44200.015.9390271
CGGGGTA19500.015.5825621
GGGGGCG13400.015.4533541
GAGGGAC31200.015.2939961
GGGGACA27800.015.137922
Produced by FastQC (version 0.11.2)