FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n02 41mh.3420000000f127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n02 41mh.3420000000f127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9527808
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13100.027.9937218
GCGGCGC11000.025.5777441
CGGGGAC8900.023.0142211
CGGGGCG4600.022.9977281
CGGGGAG10300.022.5084131
CACACGT12050.020.91102219
AGCACAC19100.020.37778717
AGAGCAC25350.019.61437815
CGGGGAT15500.019.1684551
CGGGGTT11900.018.7254891
GAGCACA26750.018.58714916
CGGGGGG5100.018.536341
CTCGGTG19450.018.50843644
CGGCGCT15350.018.3265382
GCGGGGC4550.018.3035451
CGGGGTG9100.018.05621
GGGGGAT25200.017.8638231
GGGGGAC18950.017.8166871
GAGGGAT26350.017.5967121
GGGGGCG12500.017.1063961
Produced by FastQC (version 0.11.2)