FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 44r.3410000000f0e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 44r.3410000000f0e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6888533
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG7750.010.74126640
CCCCGGA8050.09.788751
TACGGCC8450.09.31894643
CGGCCGG9700.09.04582826
CTACGGC9550.08.24556142
CCTACGG10400.08.0043141
CCCCGGG7900.07.9741877
CCCCCGA4304.089199E-67.8537641
TCGGCCG10350.07.825599725
CCGGGTA12750.07.7642229
TGCCCCG8301.8189894E-127.59308535
CCCGGGT8301.8189894E-127.58988868
CGCGCGG4303.484318E-57.32512545
CGGTCTC9850.07.3091936
GGGCTAG11200.07.231692313
CGGGTAA16200.07.22177210
GGCCGGT12250.07.162819427
ACGGCCT12300.07.13370244
TCCCTAC11600.06.982323639
CGGCCTT11950.06.966093545