FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 44mh.3410000000f187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 44mh.3410000000f187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13839976
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC22750.012.95545843
CCCTACG23100.012.85656240
CTACGGC24450.011.96264942
CGGCCTT26500.011.71645245
TAGCTTA47450.011.711845
ACGGCCT26550.011.01645144
CCTACGG29350.010.57873841
CTAGCTT72450.09.99954845
CCCCGGC13650.09.7276473
TCGGCCG13300.09.64242125
TATGTCC34050.09.44891335
ACTAGCT76750.09.43931344
TCCCTAC32400.09.37457739
TGTCCCT34850.09.10288837
CACTAGC82200.08.70398643
GCTTATC35850.08.66069643
TTCGTAC38500.08.4736427
GGGCTAG17050.08.44536613
GGCCGGT15200.08.43711927
CGGCCGG14500.08.37893126
Produced by FastQC (version 0.11.2)