FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 43r.3410000000f0cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 43r.3410000000f0cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089479
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGC14350.012.07251342
CCCTACG15000.011.39937940
TACGGCC16400.010.83782343
CGGCCTT19650.09.61779645
CCTACGG17800.09.60621841
ACGGCCT19600.09.18317344
TCCCTAC19200.09.02294639
CCCCGGA11400.08.8870251
TGCCCCG12950.08.8637185
ACCCCCC23400.08.84567242
CCCCCCC23750.08.71531543
CCCCGGG13550.08.3021317
AACCCCC27050.07.984777541
CCGGGTA22600.07.8646159
CCCCCGC3206.2942825E-47.7391171
CCCCCTC27100.07.72098245
CCCCCCT29250.07.691888344
CCTAGAT25200.07.499591424
CCCGGGT15300.07.49959148
GTCCCTA24350.07.48419238
Produced by FastQC (version 0.11.2)