FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 43mh.3410000000f16d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 43mh.3410000000f16d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10650866
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTT17550.012.69177645
CCCTACG17550.012.56357640
TACGGCC17100.012.36790243
GACAGGT29550.011.4279761
CTACGGC19500.011.30721842
ACGGCCT19850.010.88115544
CGACCGG15700.010.7507332
TGCCCCG30600.010.6613177
CGATCAG40350.010.65011144
CCTACGG21350.010.64358241
GTGCCCC30900.010.4875086
AGATCTC26100.010.43059645
TCCCTAC21800.010.42387439
GCGACCG16000.010.4123151
TCGGCCG11200.010.24510625
CCCCGGC15500.010.1634883
GGCCCCG26350.09.9963341
CGGCCGG11950.09.60210726
GGCGCCG8000.09.5680731
ACAGGTG34650.09.4176212
Produced by FastQC (version 0.11.2)