FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 42sr.3410000000f0a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 42sr.3410000000f0a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9925641
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA15400.08.9119099
CGGGTAA21100.07.997245310
CCCCGGA9000.07.50459151
CCCCGGG8553.6379788E-127.3680677
CGCGCCG8653.6379788E-127.28769541
TGCCCCG8054.1836756E-117.26889135
GCCCTCC15650.07.04904131
GGCCCCG11200.07.03555441
CCCTACG9453.8198777E-116.66634540
CCCGGGT9151.2187229E-106.63902438
GCGCCGA15300.06.6193632
TACGGCC10307.2759576E-126.553082543
GCCTCGC5958.352239E-66.4325071
CTACGGC10254.3655746E-116.365546742
GCCCCGG9654.3655746E-106.2968426
TCGGCCG9351.3969839E-96.256383426
GCCCGAC4000.0066189686.1912881
CGGCCGG9502.0136213E-96.157598527
ACTCGGG7854.5549496E-76.018818428
GACGTGT11703.274181E-115.96518851