FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 42r.3410000000f100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 42r.3410000000f100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13404520
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21700.019.18103441
TGCCGTC25200.016.60628543
GTATGCC26700.015.92614340
ATGCCGT27900.015.07986542
CTACGGC20300.014.62978642
TACGGCC20550.014.45180843
CCCTACG20800.014.38627540
GCCGTCT31350.013.20505944
TATCTCG26350.013.14922534
CGGCCTT22900.012.968760545
CCCCGGA31700.012.7838041
ACGGCCT23600.012.48876144
CCTACGG23700.012.43606641
CTCGTAT33950.012.32631437
CGCGCGG11500.012.3254545
CGTATGC36350.012.25521239
GTCACGC37350.011.44519124
CCGTCTT38000.011.30862645
ACGCGCG12450.011.2042444
TCGTATG41750.011.10122138
Produced by FastQC (version 0.11.2)