FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 42mh.3410000000f144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 42mh.3410000000f144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7711193
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC12750.016.76382643
CCCTACG13000.016.61451340
CTACGGC13950.016.28946942
CGGCCTT14300.015.26143645
ACGGCCT14950.014.59789744
CCTACGG16300.013.664927541
TCCCTAC17150.012.59409139
TGTCCCT18450.011.70670337
TATGTCC18750.011.51939535
TTCCTAG20250.011.33273922
TTCGTAC20900.010.9803567
CGCGCGG7100.010.77408345
CCTAGAT21100.010.76957824
ACGCGCG7450.010.56991644
TCGTACA21850.010.5029498
ATGTCCC21450.010.4889636
TAGATGC22100.010.18046126
CGTACAA22250.010.0107129
CTTCCTA23700.09.96783221
CCCCGGA16350.09.9150021