FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 41r.3410000000f088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 41r.3410000000f088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12052003
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGA12950.011.474231
ACCCGCG8750.08.2281657
CCCGGAA20650.07.9561762
ACCCCCC27900.07.902834442
CCGGAAC19800.07.8430263
CCCCCCC30200.07.598961443
CGCGCGG5452.7116948E-77.430824345
AACCCCC30150.07.31307141
CCCGCGC9900.07.2723678
CCCCCTC30050.07.262535645
CCGCGCT11250.07.19964369
CCCTACG16900.07.188993540
TACGGCC17250.07.173557843
CCCCCCT33100.07.00116444
GTGCCCC18800.06.9433354
CTACGGC17850.06.9324342
CGCGCTA5207.937071E-66.92733241
CTCACAC32400.06.805218718
GTACTCC34450.06.792116627
ACGGCCT19150.06.69679644