FastQCFastQC Report
Tue 18 Aug 2015
H5C72BCXX l01n01 41mh.3410000000f12a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C72BCXX l01n01 41mh.3410000000f12a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9527808
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC19900.014.24547143
CCCTACG20250.013.77704340
CGGCCTT21450.013.425858545
ACGGCCT22300.012.9141144
CTACGGC21950.012.71002942
CAGATCA58650.012.50572745
CCTACGG23750.011.84147541
TCCCTAC24650.011.40912839
TATGTCC28500.010.26261135
CGTACAA32100.09.882659
GACGTGT16450.09.8544281
CCTAGAT30850.09.77257424
TTCCTAG31550.09.76968622
TTCGTAC31750.09.7081957
GTCCCTA29600.09.65320238
ATGTCCC30950.09.59561236
TCGTACA32850.09.5200918
TGTCCCT29850.09.49698137
TGCCCCG17300.09.233667
TAGATGC33500.08.9995226
Produced by FastQC (version 0.11.2)