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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2020-09-29, 21:09 based on data in: /beegfs/mk5636/logs/html/H5C5YBGXG/merged

        General Statistics

        Showing 16/16 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        H5C5YBGXG_n01_AKM118
        78.3%
        48%
        22.4
        H5C5YBGXG_n01_AKM119
        81.4%
        49%
        22.9
        H5C5YBGXG_n01_AKM120
        79.0%
        48%
        22.5
        H5C5YBGXG_n01_AKM121
        78.8%
        49%
        22.9
        H5C5YBGXG_n01_AKM125
        75.7%
        48%
        17.3
        H5C5YBGXG_n01_AKM126
        77.9%
        48%
        23.8
        H5C5YBGXG_n01_AKM127
        76.9%
        48%
        22.5
        H5C5YBGXG_n01_AKM128
        76.7%
        48%
        22.2
        H5C5YBGXG_n01_AKM129
        75.9%
        48%
        23.1
        H5C5YBGXG_n01_AKM130
        75.6%
        48%
        27.9
        H5C5YBGXG_n01_AKM132
        75.0%
        48%
        24.0
        H5C5YBGXG_n01_AKM133
        76.9%
        48%
        25.0
        H5C5YBGXG_n01_AKM134
        77.3%
        48%
        22.5
        H5C5YBGXG_n01_AKM135
        76.9%
        48%
        24.7
        H5C5YBGXG_n01_AKM136
        78.7%
        49%
        27.1
        H5C5YBGXG_n01_undetermined
        71.2%
        44%
        12.6

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 16/16 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        12,554,337
        3.5
        AKM118
        22,434,116
        6.2
        AKM119
        22,857,525
        6.3
        AKM120
        22,482,919
        6.2
        AKM121
        22,936,093
        6.3
        AKM125
        17,311,941
        4.8
        AKM126
        23,811,526
        6.6
        AKM127
        22,515,209
        6.2
        AKM128
        22,216,020
        6.1
        AKM129
        23,076,319
        6.3
        AKM130
        27,899,089
        7.7
        AKM132
        24,026,577
        6.6
        AKM133
        25,019,139
        6.9
        AKM134
        22,508,607
        6.2
        AKM135
        24,681,115
        6.8
        AKM136
        27,125,020
        7.5

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGG
        8665960.0
        69.0
        CCCGTC
        66098.0
        0.5
        GTCCCG
        55594.0
        0.4
        AATAAA
        52182.0
        0.4
        GGGGGC
        48886.0
        0.4
        GGCCAA
        47713.0
        0.4
        AATTAT
        45974.0
        0.4
        AATTCA
        45130.0
        0.4
        CAGTCA
        44915.0
        0.4
        ACAAAA
        44456.0
        0.3
        AATGAA
        38221.0
        0.3
        GTTCGG
        36892.0
        0.3
        GACAAA
        36190.0
        0.3
        ACAAAT
        35929.0
        0.3
        CATAAC
        35546.0
        0.3
        ATTAAA
        35264.0
        0.3
        GTGGGG
        34206.0
        0.3
        GGGGCG
        34039.0
        0.3
        ACTAAA
        33506.0
        0.3
        GGGCGG
        33336.0
        0.3

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        4.0
        385,399,320
        363,455,552
        3.5
        1.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (76bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.