FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12554337
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT197650.053.14017512
GCACACG201750.052.11139711
CGTCTGA188500.051.6702116
ACGTCTG190150.051.5529915
CACGTCT190500.050.979114
CTGAACT194300.050.69326419
ACACGTC203300.048.8804913
AGCACAC234350.046.7241410
GATCGGA258000.044.3179321
AGAGCAC259300.043.861678
TCTGAAC230650.043.19610618
TCGGAAG267050.043.0623663
GTCTGAA237200.042.85878417
CGGAAGA272700.042.794024
CCAGTCA182650.042.4816326
ATCGGAA276900.042.0473822
CTCCAGT223100.041.35375624
CAGTCAC150200.040.99393527
AACTCCA259550.040.8041822
GAACTCC248000.040.79183621
Produced by FastQC (version 0.11.5)