FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27125020
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT7348602.709159292785775TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG796100.067.3917148
ATGCCGT804800.066.68432649
TATATCT817650.065.44417639
TATCTCG822950.065.0606241
GTATGCC829550.064.7358547
ATATATC843350.063.56418638
TGCCGTC851050.063.1036150
GATATAT854700.063.08021537
ACTGATA857350.063.02380834
CGTATGC854050.062.95278546
TGATATA859450.062.80512236
ACACTGA871150.062.26294732
GCCGTCT865600.061.9915151
CTCGTAT872050.061.63250444
GTCACAC884500.061.3618629
CTGATAT882200.061.34058835
CACTGAT884350.061.19632333
CACACTG888750.060.918931
TCGTATG902600.059.82222745
TCTCGTA903500.059.3647443
Produced by FastQC (version 0.11.5)