FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24681115
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT6881212.788046650242503TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG741300.067.8820948
ATGCCGT748750.067.1824749
TATCTCG766250.065.4879941
GTATGCC773050.065.13734447
TGCCGTC786400.063.9590650
CGTATGC795350.063.3547846
GCCGTCT796700.063.09899551
CTCGTAT801350.062.7745844
TCGTATG832750.060.62230345
TCTCGTA840150.059.89354343
ACGAGTG848650.059.8495732
ATATCTC841050.059.7561240
GATATCT845550.059.33425539
CACGAGT866850.058.58812731
TGGATAT865100.058.34942237
GTGGATA882150.057.19796836
CTTGAAA882600.057.1352362
TCACGAG891400.057.0097830
GTCACGA892400.056.97000529
AGTGGAT888100.056.8699335
Produced by FastQC (version 0.11.5)