FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22508607
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT4212311.8714218965216283TruSeq Adapter, Index 22 (97% over 40bp)
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA287540.1277466881891003No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG257430.11436958315545692No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG230760.10252078238337893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG455800.067.96892548
ATGCCGT462900.066.88785649
TACGTAA481500.064.72349536
CGTACGT482600.064.3364934
GTATGCC484850.064.0369747
GTACGTA487650.063.87880335
ACCGTAC495150.062.9579232
TGCCGTC498100.062.15700550
GTAATCT497950.061.6653639
CCGTACG507700.061.2433633
CTCGTAT505350.060.9257344
CGTATGC513700.060.39301346
GCCGTCT513300.060.19738851
CACCGTA526600.059.30996331
GTCACCG536400.058.13464429
TCACCGT543750.057.76719330
TCTCGTA541600.056.7870843
TCGTATG549750.056.62261645
AGTCACC558150.056.01874528
ACGTAAT563350.055.3070437
Produced by FastQC (version 0.11.5)