FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25019139
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT2485270.9933475328627416TruSeq Adapter, Index 21 (97% over 40bp)
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG254150.10158223270592966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG252250.067.10268448
ATGCCGT252750.064.8915449
GTATGCC286000.060.7413947
CGTTTCG435950.056.60700233
TGCCGTC286250.056.50529550
CGTATGC305750.056.25715346
GTTTCGG439550.056.08124534
CTCGTAT332150.055.0511944
GCCGTCT297350.051.6387651
TTTCGGA480500.051.41177735
CACGTTT489750.050.729331
TCGTATG344450.050.45395345
GTCACGT495000.050.1912629
ACGTTTC498250.049.8019532
CGGAATC488250.049.54273238
TCTCGTA380650.048.51352743
TCGGAAT508800.048.32567237
CGTCTGA533850.046.77253716
CAGTCAC539150.046.15250427
CTGAACT546350.045.85578519
Produced by FastQC (version 0.11.5)