FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24026577
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT3206081.3343889976503935TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG344350.067.328248
ATGCCGT352300.065.7091149
TATCTCG368750.062.31363741
GTATGCC375500.061.8204547
CGTATGC387500.059.8876946
CTTATCT384700.059.594739
TGCCGTC388200.059.5551650
CGTGGCC392350.059.22287833
GCCTTAT394350.058.53481337
GGCCTTA404900.057.13942736
GCCGTCT404950.057.0895151
TTATCTC403900.056.8917640
CTCGTAT410100.056.4338644
TCGTATG425150.054.75699645
GTGGCCT435150.053.2232834
TCTCGTA438750.052.57905243
GTCACGT462450.050.63739829
TCACGTG480950.048.7326630
CCTTATC476400.048.358338
CTTGAAA490450.047.30056462
Produced by FastQC (version 0.11.5)