FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27899089
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT3393171.2162296768901666TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG400550.066.9621748
ATGCCGT410450.065.3219449
GTATGCC432000.062.1062547
CCGCACA421250.062.07502736
TGCCGTC449450.059.6946450
CGTATGC453650.059.15000546
CGCACAT450650.058.3751337
CTCGTAT461700.057.79228244
GCCGTCT468350.057.23736651
TCGTATG488000.055.0362945
TCCGCAC484800.054.16153735
CACATCT495900.053.09856839
GCACATC497300.052.9202738
TCTCGTA508600.052.43937743
ACATCTC530950.049.90334740
CGTCCGC535900.049.43170533
GTCACGT553300.048.84349429
TCACGTC551100.048.74609430
GTCCGCA550250.048.0810434
CTTGAAA565550.047.56074562
Produced by FastQC (version 0.11.5)