FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23076319
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT3756071.627672940385336TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG424850.067.4814748
ATGCCGT431400.066.4486349
CGTCCCG429800.066.0439435
CCGTCCC435400.065.2430234
CACCCGT445650.064.2874531
CCCGTCC444550.064.157633
TCACCCG447150.064.0722230
CCCGATC437200.063.27746238
GTATGCC454750.063.13922547
GTCCCGA450050.063.01075736
GTCACCC454950.062.96588529
ACCCGTC460050.062.1924632
TGCCGTC463550.061.8954250
CGTATGC466350.061.63624646
CTCGTAT471750.059.75020244
GCCGTCT483350.059.3137851
AGTCACC493300.058.41819828
TCCCGAT488250.057.73653437
TCGTATG502000.057.35621345
TCTCGTA508100.054.95015743
Produced by FastQC (version 0.11.5)