FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22216020
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT4308781.9394923123043644TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG454650.067.2777348
ATGCCGT455350.066.2133249
GTATGCC492550.063.24149747
TGCCGTC478250.062.7959850
CGTATGC497050.062.29570846
TGTCAGA631200.060.991235
ATGTCAG633350.060.83913834
GTCACAT639750.060.73412729
CACATGT640100.060.57557331
GCCGTCT477800.060.48644651
CTCGTAT540600.060.28066644
AGTCACA660450.059.0306228
ACATGTC660950.058.73987232
GTCAGAA663700.058.27868736
TCGTATG537450.058.2506945
CATGTCA671250.057.5792733
TCTCGTA576700.056.9708843
TCAGAAT679700.056.5771237
CAGTCAC694100.056.03834527
CACACGT707250.055.4791112
Produced by FastQC (version 0.11.5)