FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22515209
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT4531322.0125595991580623TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG502450.067.31969548
ATGCCGT507950.066.3831749
TATCTCG528500.064.06827541
GTATGCC530600.063.994847
TGCCGTC533450.063.16749250
CGTATGC539950.062.84126346
CGTATCT551500.061.44820839
GCCGTCT547900.061.1609351
CTCGTAT560700.060.5017144
GTCACAG574900.060.45096229
TCACAGT581050.059.82906730
TCGTATG571850.059.3776645
GTATCTC577500.058.81530840
AGTCACA605750.057.63214528
TCTCGTA592700.057.23972343
CACAGTT624500.055.7671731
CAGTCAC644400.054.16460427
CGTCTGA649550.054.03927216
CTTGAAA630100.053.4369662
GTCTGAA657900.053.3476217
Produced by FastQC (version 0.11.5)