FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23811526
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT5611962.35682500987127TruSeq Adapter, Index 13 (97% over 40bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA259120.1088212490035288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG625550.067.7547448
ATGCCGT632450.066.92473649
GTATGCC652700.065.0298747
TGCCGTC657800.064.3278650
CGTATGC665400.063.8148646
GCCGTCT680750.062.11595551
CTCGTAT688400.061.0306344
GTCACAG703650.060.7187629
TCACAGT709000.060.32943330
TCGTATG707600.060.18688645
TCTCGTA718050.058.39084243
AGTCACA741050.057.87110528
CACAGTC739950.057.73047331
AGTCAAC748450.056.8551734
ACAGTCA760600.056.18742432
CTTGAAA761900.055.85702562
GTCAACA769750.055.55076635
AACAATC764000.054.92433538
CAGTCAC785950.054.48064827
TTCTGCT785850.053.85341657
Produced by FastQC (version 0.11.5)