FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17311941
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC5269063.0435986351848126TruSeq Adapter, Index 12 (100% over 50bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA187090.10806991544160184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG590750.068.3576546
ATGCCGT595250.067.69974547
GTATGCC609150.066.3723245
TGCCGTC612050.065.7408448
CGTATGC624600.064.724244
GCCGTCT626750.063.96885749
TGTAATC619250.063.48431836
GTAATCT619200.063.35341637
CTCGTAT621250.063.08329442
TCGTATG643150.062.78428343
GTCACCT661700.061.71954729
TTGTAAT660400.061.59039735
AGTCACC666850.061.29956428
TCTCGTA642200.061.0257341
CTTGTAA668100.060.7962134
TAATCTC648250.060.53543538
CTTGAAA679850.059.247160
CACCTTG699150.058.42822331
ACCTTGT698450.058.38791732
AAAAGGG564850.058.1649970
Produced by FastQC (version 0.11.5)