FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22936093
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC3738671.630037862159TruSeq Adapter, Index 8 (100% over 50bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG346730.15117221577362805No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA312720.1363440582491534No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA275030.11991144263323313No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT232090.10118985827272325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG421400.067.74464446
ATGCCGT415400.066.6503247
GTCACAC567800.063.66547829
GTATGCC459250.063.4565845
TGCCGTC434750.062.3697448
ACACTTG589850.060.8174432
GCCGTCT437050.060.20639849
CGTATGC480850.060.13281244
CGTCTGA613550.059.20549416
CACACGT616450.059.1087612
GCACACG625450.058.26342411
ACGTCTG624350.058.19804815
CTCGTAT491000.058.139242
CACGTCT629100.057.75255614
CTGAACT637350.057.07700319
AAAAGGG481250.056.91611570
AGTCACA638200.056.73525628
GTCTGAA645250.056.38348417
TCTGAAC646150.056.3101218
TCGTATG505250.055.65382843
Produced by FastQC (version 0.11.5)