FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22482919
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC7047243.1344862293014533TruSeq Adapter, Index 7 (100% over 50bp)
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA359430.15986803137083755No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG324640.14439406199880006No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA246130.10947421907271027No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA232580.10344742157368444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG796100.067.8380346
ATGCCGT795050.067.3031947
GTATGCC830650.065.4540345
CAGATCA889150.064.9765334
TGCCGTC819900.064.8922948
TCACCAG913000.063.90530430
GCCGTCT821400.063.83604449
AGTCACC916400.063.5956428
CGTATGC858300.063.28343244
CTCGTAT844250.063.1128442
GTCACCA935300.062.3034729
TCTCGTA877200.060.7897641
TCGTATG881650.060.771843
AAAAGGG763900.060.60542370
CGTCTGA970800.060.43572216
CACACGT985400.059.84216712
CTGAACT979800.059.8266219
ACCAGAT973850.059.8192132
ACGTCTG982750.059.7012415
AGATCAT967500.059.67494635
Produced by FastQC (version 0.11.5)