Basic Statistics
Measure | Value |
---|---|
Filename | H5C5YBGXG_n01_AKM119.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22857525 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 308210 | 1.3483962065009225 | TruSeq Adapter, Index 6 (100% over 50bp) |
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG | 41418 | 0.1812007205504533 | No Hit |
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA | 32724 | 0.1431651064583764 | No Hit |
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA | 32443 | 0.14193575201164604 | No Hit |
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA | 28524 | 0.1247904136602716 | No Hit |
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA | 27976 | 0.12239295374280462 | No Hit |
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG | 23325 | 0.1020451689323319 | No Hit |
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGA | 23085 | 0.10099518648672592 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 33745 | 0.0 | 67.8574 | 46 |
ATGCCGT | 34455 | 0.0 | 66.47926 | 47 |
TCACGCC | 35735 | 0.0 | 64.178604 | 30 |
TATCTCG | 36065 | 0.0 | 63.334885 | 39 |
CACGCCA | 36765 | 0.0 | 62.484478 | 31 |
GTCACGC | 37155 | 0.0 | 61.95241 | 29 |
GCCAATA | 37240 | 0.0 | 61.608295 | 34 |
GTATGCC | 37380 | 0.0 | 61.361107 | 45 |
TGCCGTC | 37485 | 0.0 | 61.14974 | 48 |
CGCCAAT | 37445 | 0.0 | 61.14615 | 33 |
GCCGTCT | 38220 | 0.0 | 59.807503 | 49 |
CGTATGC | 39805 | 0.0 | 57.763813 | 44 |
CTCGTAT | 40195 | 0.0 | 56.800415 | 42 |
CCAATAT | 42090 | 0.0 | 54.767723 | 35 |
ATATCTC | 42260 | 0.0 | 54.249 | 38 |
TCGTATG | 43465 | 0.0 | 52.93001 | 43 |
TCTCGTA | 43715 | 0.0 | 52.28269 | 41 |
AATATCT | 44155 | 0.0 | 52.062912 | 37 |
CGTCTGA | 44930 | 0.0 | 51.99249 | 16 |
CACACGT | 45530 | 0.0 | 51.530354 | 12 |