FastQCFastQC Report
Tue 29 Sep 2020
H5C5YBGXG_n01_AKM119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C5YBGXG_n01_AKM119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22857525
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3082101.3483962065009225TruSeq Adapter, Index 6 (100% over 50bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG414180.1812007205504533No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA327240.1431651064583764No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA324430.14193575201164604No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA285240.1247904136602716No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA279760.12239295374280462No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG233250.1020451689323319No Hit
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGA230850.10099518648672592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG337450.067.857446
ATGCCGT344550.066.4792647
TCACGCC357350.064.17860430
TATCTCG360650.063.33488539
CACGCCA367650.062.48447831
GTCACGC371550.061.9524129
GCCAATA372400.061.60829534
GTATGCC373800.061.36110745
TGCCGTC374850.061.1497448
CGCCAAT374450.061.1461533
GCCGTCT382200.059.80750349
CGTATGC398050.057.76381344
CTCGTAT401950.056.80041542
CCAATAT420900.054.76772335
ATATCTC422600.054.24938
TCGTATG434650.052.9300143
TCTCGTA437150.052.2826941
AATATCT441550.052.06291237
CGTCTGA449300.051.9924916
CACACGT455300.051.53035412