H5C5YBGXG_n01_AKM118.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5C5YBGXG_n01_AKM118.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22434116
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC	301468	1.3437926415286432	TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC	46608	0.20775501027096407	TruSeq Adapter, Index 5 (98% over 50bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG	43620	0.1944360098699677	No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA	33377	0.1487778702757889	No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA	32274	0.14386125131919617	No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA	24669	0.10996198825039506	No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA	23623	0.10529944661068884	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	39980	67.68469	46
ATGCCGT	40505	66.349556	47
GTATGCC	42875	63.424583	45
TGCCGTC	43380	61.554417	48
CACACAG	46210	60.817524	31
CACAGTG	46255	60.698914	33
GTCACAC	46830	60.206512	29
GCCGTCT	43945	60.181717	49
ACACAGT	47030	59.80258	32
CGTATGC	46075	58.912735	44
TCACACA	48595	58.170635	30
CTCGTAT	41040	56.56078	42
TCGTATG	48785	54.841427	43
AAAAGGG	42075	54.773567	70
CGTCTGA	53440	53.128365	16
ACAGTGA	53565	52.384544	34
TCTCGTA	44260	52.22445	41
ACGTCTG	54440	52.22282	15
CTGAACT	54675	51.87116	19
CACACGT	55275	51.60671	12