FastQCFastQC Report
Wed 24 Mar 2021
H5C3VDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5C3VDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060855104
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAGCGA563816945.314740324801227No Hit
CTGGACTC511011164.816974137874346No Hit
CCCAGGAT501959434.7316492903445555No Hit
GCCTTGGT501746264.729639873608979No Hit
CTGTAACT481446194.538284146295628No Hit
GACCCTAC462000524.354982299260352No Hit
CTAAGTTT459646784.332795103373514No Hit
TACCACCA454641414.285612693814215No Hit
AGGTACGC448759534.230167987201389No Hit
GCTACCTG436123624.111057375843101No Hit
TGCTGTAA435781624.1078335614059505No Hit
CTGCAAGC434453994.0953188457299445No Hit
AAACCTCA430524144.058274672730424No Hit
TGTAGAAG428265484.036983734962546No Hit
GGGTCAAG422463783.9822948337344286No Hit
AAAGTGCT417727113.9376452865706337No Hit
ACTGTGGC398015133.7518331061354817No Hit
GTTCCTCA397325963.745336742990304ABI Solid3 Adapter A (100% over 8bp)
TAAGTATG390287913.6789935640447275No Hit
AGAGTGTG377568333.559094249312298No Hit
TGACGCCC283823572.675422580612856No Hit
ACCGTATG245340972.3126718161125988No Hit
CTGACTGA243289732.2933360935217784No Hit
GGGGGGGG236067242.2252543171060615No Hit
GATTAGAT219786652.0717876472600727No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)