FastQCFastQC Report
Tue 4 Jun 2024
H57WGDRX5_n02_R01.Leg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57WGDRX5_n02_R01.Leg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33553266
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGATCGTCGCAAGACGGACCGAAGCGAAAGCATTTGCCAAGTATGTT943280.28112911571708105No Hit
CCGGTTCCTATTCCGGAACCCGGCTGCGGAACCGTCACTTCGCGGGCACT811090.24173205672437373No Hit
GGGGAAGCCGGCCACGGCCGGCAGCCCCGAGGGAAGACGCGGCCGTCGCC806580.2403879252767823No Hit
CTAACGTGTGCGCGAGTCGTTGGGACAGCGCATGTCGAATCCCGTGGGCG664030.19790323839115992No Hit
CTTGGCTCGTGGGTCGATGAAGAACGCAGCAAAATGCGTGACTATATGTG543130.1618709785211371No Hit
GTCTAACGTGTGCGCGAGTCGTTGGGACAGCGCATGTCGAATCCCGTGGG524340.15627092754547353No Hit
CCGGGATTCCCTTAGTAGCTGCGAGCGAACAGGGAAAAGCCCAGCGCCGA507340.15120435667872095No Hit
TGCAAACAGAGTCCCGACCAGAAATGGAAGGGACGCTTTTATTAGATCAA500360.1491240822875484No Hit
GGGAAGCCGGCCACGGCCGGCAGCCCCGAGGGAAGACGCGGCCGTCGCCC498420.14854589714157782No Hit
CGACTGTCTAATTAAAGCAAAGCATTGCGATGGCCCCAGCTGGGTGTTTA496460.14796175132399927No Hit
CTGGAATCCTATAGCAGGGAGATAGGGTTTGGAACGCGAAGAGCACCGCA483610.1441320198158951No Hit
GACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGT479570.1429279641510904No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC475980.14185802359746438No Hit
CACGTTTTTACCGCACGTCGGCACGTCGGCTGCCAGATGGCGTCGCCCGA473780.14120234972059054No Hit
CTCGGTTCTATTTTGTTGGTTTTCGGAATCTGAGGTAATGATCAATGGGG471590.14054965617952064No Hit
GCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAACCAACCGGGATT455620.1357900599005772No Hit
CCGGAATGTCGAAAGGAGAGATTCACGTTTTTACCGCACGTCGGCACGTC447910.1334922209957147No Hit
CAAATACTCTAGCGAGGGCCTGGAGGGCTGATGTGGAGAAGGGTTTCTTG418480.12472109272462478No Hit
CTTTATTAGGCGTAGCGCCTCGCGGGGCGCGCGTCGCAAGCCCAATGGCC409130.12193447874791086No Hit
GTTAGTTTTACCCTACTGATGACACGTCGTTGCGATAGTAATCCTGCTCA406610.12118343412530988No Hit
GGGAATCCGGTTCCTATTCCGGAACCCGGCTGCGGAACCGTCACTTCGCG402140.11985122402093436No Hit
GGAAAAGATCCGGAATGTCGAAAGGAGAGATTCACGTTTTTACCGCACGT398640.11880810648954411No Hit
CGACGCTCATGGGAAACCATGAAAGGCGTTGGTTGCTGAAGACAGCAGGA397690.11852497458816676No Hit
ATTTGGTTCACGCACTCGGTCGAGCGGCCGATGGTGCGAAGCTACCATCC396830.11826866570902517No Hit
CCCTGATGGAGGTCCGCAGCGATTCTGACGTGCAAATCGATCGTCTGAGC391190.1165877563155849No Hit
CGAGACTCTAGCCTACTAACTAGGCGTACCCGGTATCCACAATCGTGCTA388660.11583373135717996No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG388340.11573836061145285No Hit
GTGGGATTACCCGCTGAATTTAAGCATATCACTAAGCGGAGGAAAAGAAA387050.11535389729274044No Hit
CGAGATTCTCGCTGTCCCTATCTACTATCTAGCGAAACCACTGCCAAGGG383020.11415282196373967No Hit
CGCATGTCGAATCCCGTGGGCGTAATGAGAGTGAAGGGGAAGCCGGCCAC375570.11193247178978047No Hit
CTTAGATGTCCTGGGCCGCACGCGCGCTACACTGAAGGAATCAGCGTGTC374900.11173278929091433No Hit
GAGAAATCCGTCGGCTTACCGGGATCCGAGCTCGGTCTCGTGCCTTGGCC373900.11143475571051711No Hit
CTTGGATCGTACAATCCATTACTTGGATAACTGTGGTAATTCTAGAGCTA365750.10900578203027986No Hit
GGGAAGTCGGCAAACTGGATCCGTAACTTCGGAACAAGGATTGGCTCTGA363190.10824281606446298No Hit
AGGGAATCCGGTTCCTATTCCGGAACCCGGCTGCGGAACCGTCACTTCGC360570.1074619680838223No Hit
CGGGGAGGTAGTGACGAAAAATAACGATACGGGACTCATCCGAGGCCCCG344920.10279774255060595No Hit
CGGCGGTCCTCCCCGGGTCCGTCGGGTACCTCGAGCCTGCGTTATTACCG337120.10047308062350771No Hit
CGAGAGTCGAAAGGCTCGATTAATATGCTACTTTATTAGGCGTAGCGCCT335710.10005285327514765No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)