FastQCFastQC Report
Tue 4 Jun 2024
H57WGDRX5_n02_H04.Leg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57WGDRX5_n02_H04.Leg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40062708
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAAGTAGCAACTAAAAACTCAAAACTAAACAATGGGCAAGGAAAGGA1108880.27678608245852976No Hit
CTTGAAGTAGCAACTACAAACTCAAAACTCAACAATGGGCAAGGAAAGGA783830.19565077827489843No Hit
ATTGAAGTAGCAACAACAAACTCAAAACTCAACAATGGGCAAGGAAAGGA769740.19213379185450968No Hit
CAAACTCAAAACTCAACAATGGGCAAGGAAAGGAACTGCATCAACATCGC753760.18814504501293322No Hit
CAACAACAAACTCAAAACTCAACAATGGGCAAGGAAAGGAACTGCATCAA734060.1832277538502889No Hit
CGAAGTAGCAACAACAAACTCAAAACTCAACAATGGGCAAGGAAAGGAAC664280.16581005956961273No Hit
GGAAAGGAACTGCATCAACATCGCCGTTGTCGGCTCACTCTGATTTATCT618300.15433305207426318No Hit
GCAACAACAAACTCAAAACTCAACAATGGGCAAGGAAAGGAACTGCATCA536830.13399743222550006No Hit
GCTTGAAGTAGCAACTACAAACTCAAAACTCAACAATGGGCAAGGAAAGG509320.12713069720599018No Hit
AACAAACTCAAAACTCAACAATGGGCAAGGAAAGGAACTGCATCAACATC509290.1271232089453364No Hit
CAAACTCAAAACTCAACAATGGGCAAGGAAAGGAATTGCATCAACATCGC452050.11283560761793736No Hit
TAAAAACTCAAAACTAAACAATGGGCAAGGAAAGGAACTGCATCAACATC412630.10299603311887953No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)