FastQCFastQC Report
Tue 4 Jun 2024
H57WGDRX5_n01_R01.Leg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57WGDRX5_n01_R01.Leg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33553266
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCTCGGGATAGAATCCCAAAGGACCGCAATGTGC1140850.340011610196158No Hit
CCGACATCGAACTCCCCGTAGGGCTACCAATGTTACGCCGTTCGGCGGGG1122860.3346499860848121No Hit
CCAACGTGTACGCTCTGGGTGCGCCTCGCCTCGCCTTGCGGAGAGATCGA898740.2678547000461893No Hit
CCTCGCTAGAGTATTTGCTACTACCACCAAGATCTGCACCGACGGCGGCT717560.2138569759498226No Hit
GTCGGATTCCCCCGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGG713150.21254264786027088No Hit
GTCGCAGACAGGTTCACGCGGGAACGGACGGAGACCTTACCGCCAACAGC709710.2115174123437045No Hit
ATCAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTTGTGCAA615350.18339496369742367No Hit
GGCGGTTTCACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT615230.18335919966777603No Hit
TATTAATCGAGCCTTTCGACTCTCGGGACTCCTGGTGATATCGTCGCAAC574320.17116664589372613No Hit
CCCCAGTCAAACTCCCCGCCTGGCTGTGTCCTCGAATCGGATCACGCGAG557870.166263993496192No Hit
CTTCAGTGTAGCGCGCGTGCGGCCCAGGACATCTAAGGGCATCACAGACC551500.16436551958906176No Hit
TCAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTTGTGCAAC547480.16316742459586497No Hit
GTCGTATTTAGCCTTAGATGGAGTTTACCACCCACTTAGGGCTGCACTCT541350.16134047874803006No Hit
CAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTTGTGCAACG524180.15622324217260997No Hit
GGCAGGCATAGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGA521250.15535000378204614No Hit
CGTCACTACCTCCCCGTGCCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT500850.14927011874194304No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA478700.14266867493614482No Hit
CTGGCTGTGTCCTCGAATCGGATCACGCGAGGGAGTGTTTGCCGGTCGGA463510.13814154484991117No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC458990.13679443306651579No Hit
CGAGTAAGTAAGGAAACGATGAAAGTAGTGGTATTTCACCGTCGAACGGG451590.13458898457157642No Hit
CGGGTGAACAATCCGACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAG448530.13367700181556097No Hit
CGGGACTCCTGGTGATATCGTCGCAACCTTGCCTAGACGGGATTCGGCCT442100.13176064589360692No Hit
CGCAGACAGGTTCACGCGGGAACGGACGGAGACCTTACCGCCAACAGCGC434600.12952539404062782No Hit
CCGCCGTTTACCCGCGCTTGCTTGAATTTCTTCACTTTGACATTCAGAGC427460.12739743427659175No Hit
CTCCAATTGATACTCGTATAAGGATTTAGAGTATACTCATTCCGATTACG423470.12620828029080686No Hit
GCCGTGCTTTCGCAGTCCCTATGCGTACTGGACATCGGGATCAAACCAGC421560.1256390361522482No Hit
CTCCACATCAGCCCTCCAGGCCCTCGCTAGAGTATTTGCTACTACCACCA417220.1243455704133243No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGATACTC416220.12404753683292709No Hit
CATCAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTTGTGCA416120.12401773347488737No Hit
CTCCTACTCGTCAAGGCTTAACGGCCGAGCCGGCCGGAAAAACCGGCGGG408580.1217705602786924No Hit
CCCGCCTGTCCCCATTGATCATTACCTCAGATTCCGAAAACCAACAAAAT408290.12168413054037722No Hit
CTAAGAAGAAATTTGACCGCCGGTAGCACGATTGTGGATACCGGGTACGC407760.12152617274276667No Hit
GTCAAACTCCCCGCCTGGCTGTGTCCTCGAATCGGATCACGCGAGGGAGT405270.12078406912757761No Hit
GTCGATTCGGGCGCCTTAACCGGGCGTTTGGTTCATCCCACAGCGCCAGT400350.11931774391202336No Hit
CTGCCCATCAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTT397840.11856967962522635No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAGCTCTGGTTTCCCGG393690.11733284026657792No Hit
CACAGATCCAACTACGAGCTTTTTAACCGCAACAACTTTAATATACGCTA386760.11526746755442525No Hit
GCAGAAATCACATTGCGTAAACACCCAGCTGGGGCCATCGCAATGCTTTG378140.11269841809140128No Hit
CTCGAATCGGATCACGCGAGGGAGTGTTTGCCGGTCGGACACGCCCGGTA374400.11158377250071573No Hit
GCCAGGCATAGTTCACCATCTTTCGGGTCCCAACGTGTACGCTCTGGGTG370350.11037673650010703No Hit
CTTTTCTGACACCTCTTGCTGAAATCTCTTCAAGCCAAAAGGATCGACAG369290.11006082090488598No Hit
GGCAGAAATCACATTGCGTAAACACCCAGCTGGGGCCATCGCAATGCTTT366570.10925016956620556No Hit
CCGGAACGCTGCGCCAACCCGGTCGGCCGCCAGCAACCCTCGGACGCTTG347850.10367098094116979No Hit
CCCGCAGCAAGGAAGATCCGCGGGAGGCCAAGGCACGAGACCGAGCTCGG345320.10291695598276483No Hit
AGACATTTGAAGGATGCGTCGCCGGTTCGAAGACCGTGCGATCTGCCAAA340150.10137612237211126No Hit
CCCGGAACGCTGCGCCAACCCGGTCGGCCGCCAGCAACCCTCGGACGCTT340130.10137016170050331No Hit
GTCACTACCTCCCCGTGCCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT337380.10055056935441098No Hit
CCTGGCTGTGTCCTCGAATCGGATCACGCGAGGGAGTGTTTGCCGGTCGG337070.10045817894448784No Hit
GTCGTTTCGGCCCCAAGGCCTCTAATCATTCGCTTTACCGCATGAGACTC335970.10013034200605092No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)