FastQCFastQC Report
Tue 4 Jun 2024
H57WGDRX5_n01_H04.Leg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57WGDRX5_n01_H04.Leg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40062708
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAAGTATGTGAATTTACAATTGAATTGTATCTACTTATTTTTTTTT1059880.26455525672403374No Hit
GCCAGTCATTCCGTGTCCAAAAATCATTTTTTAACGATCCATAATTTCAA599410.14961794394927072No Hit
GCCGAAAAGTATGTGAATTTACAATTGAATTGTATCTACTTATTTTTTTT566290.14135090418750526No Hit
GCCAGTCATTCCATGTCCAAAAATCATTTTTTAACGATCCATAATTTCAA554990.1385303260079174No Hit
GGCGGTACCATTCTGTGTCCAAAAATCATTTGAGTTGTAGTATATCCATG550270.13735217299839042No Hit
GCCAGTCATTCCGTGTCCAAAAATCATTTGTTAACGATCCATAATTAAAG481860.12027644262090322No Hit
CGAAAAGTATGTGAATTTACAATTGAATTGTATCTACTTATTTTTTTTTT465160.1161079775236362No Hit
CCAAAATCAATTTTTTTAACGATCCATAATTTCAAGCCAGATAAATCAGA414790.1035351878859512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)