FastQCFastQC Report
Tue 4 Jun 2024
H57WGDRX5_n01_C03.Head.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57WGDRX5_n01_C03.Head.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61600648
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTATTTAGCCTTAGATGGAGTTTACCACCCACTTAGGGCTGCACTCT4211130.6836178086957787No Hit
CCTCGCTAGAGTATTTGCTACTACCACCAAGATCTGCACCGACGGCGGCT2683440.43561879413995774No Hit
GGCGGTTTCACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT2664580.43255713803530116No Hit
GGCAGGCATAGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGA2310020.374999301955395No Hit
GTACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGCGCTTACT2211330.3589783665912086No Hit
CTCCAATTGATACTCGTATAAGGATTTAGAGTATACTCATTCCGATTACG2203230.3576634453585618No Hit
CCAACGTGTACGCTCTGGGTGCGCCTCGCCTCGCCTTGCGGAGAGATCGA2138640.3471781660478636No Hit
CTGGCTGTGTCCTCGAATCGGATCACGCGAGGGAGTGTTTGCCGGTCGGA1957480.3177693845038773No Hit
CCCCATTGATCATTACCTCAGATTCCGAAAACCAACAAAATAGAACCGAG1831730.29735563820692273No Hit
CAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGCGCTTACTGGG1724630.27996945746414875No Hit
CTCGTGGTCGTATTTAGCCTTAGATGGAGTTTACCACCCACTTAGGGCTG1680490.2728039484259971No Hit
CGGGACTCCTGGTGATATCGTCGCAACCTTGCCTAGACGGGATTCGGCCT1607990.2610345917140352No Hit
CCCCAGTCAAACTCCCCGCCTGGCTGTGTCCTCGAATCGGATCACGCGAG1533200.24889348566593003No Hit
CCGACATCGAACTCCCCGTAGGGCTACCAATGTTACGCCGTTCGGCGGGG1524490.2474795395009481No Hit
GGCAGAAATCACATTGCGTAAACACCCAGCTGGGGCCATCGCAATGCTTT1449160.2352507720373331No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACT1386520.22508204783819805No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCG1369870.22237915419331306No Hit
AGCATATTAATCGAGCCTTTCGACTCTCGGGACTCCTGGTGATATCGTCG1369640.22234181692374405No Hit
CTCGGGACTCCTGGTGATATCGTCGCAACCTTGCCTAGACGGGATTCGGC1365330.22164214895921225No Hit
GTCGCAGACAGGTTCACGCGGGAACGGACGGAGACCTTACCGCCAACAGC1312290.2130318499246956No Hit
TATTAATCGAGCCTTTCGACTCTCGGGACTCCTGGTGATATCGTCGCAAC1270020.2061699091217352No Hit
CCCCGGAACGCTGCGCCAACCCGGTCGGCCGCCAGCAACCCTCGGACGCT1254660.20367642885834578No Hit
CCCGGAACGCTGCGCCAACCCGGTCGGCCGCCAGCAACCCTCGGACGCTT1238040.2009784052921002No Hit
CTAAGAAGAAATTTGACCGCCGGTAGCACGATTGTGGATACCGGGTACGC1210800.19655637388749547No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1199800.19477067838637022No Hit
CTTCAGTGTAGCGCGCGTGCGGCCCAGGACATCTAAGGGCATCACAGACC1193200.19369926108569507No Hit
GGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGCGC1187900.192838880526062No Hit
GTCGGATTCCCCCGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGG1184610.19230479523527091No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGC1178540.19131941599055904No Hit
CGGGACTCTGTTTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAG1177180.19109863909223812No Hit
CTGGGCGGTTTCACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC1150250.18672693183357422No Hit
CGTCACTACCTCCCCGTGCCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT1140640.18516688330940934No Hit
ATCAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTTGTGCAA1126290.18283736236021414No Hit
CTCAGACAGGCGTAGCCCTCGGGATAGAATCCCAAAGGACCGCAATGTGC1099840.17854357635978113No Hit
GCAGAAATCACATTGCGTAAACACCCAGCTGGGGCCATCGCAATGCTTTG1099830.17854195300023468No Hit
CTCGCTAGAGTATTTGCTACTACCACCAAGATCTGCACCGACGGCGGCTC1083580.17590399373720872No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC1079360.17521893600859523No Hit
CTGCCCATCAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTT1069720.17365401740579092No Hit
CTCCTACTCGTCAAGGCTTAACGGCCGAGCCGGCCGGAAAAACCGGCGGG1059600.1720111775447557No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1025370.16645441781716322No Hit
CATCAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTTGTGCA998850.1621492682999049No Hit
CCCTGGGCGGTTTCACGTACTCTTGAACTCTCTCTTCAAAGTTCTTTTCA993420.16126778406616762No Hit
CTTGCCTAGACGGGATTCGGCCTTAGAGGCGTTCAGGCATAATCCCACGG968530.15722724215498513No Hit
GCCAGGCATAGTTCACCATCTTTCGGGTCCCAACGTGTACGCTCTGGGTG967990.15713958073947534No Hit
GTTTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAATGGATT945950.15356169629903893No Hit
CTCAGATTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATT932060.1513068498889817No Hit
ACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGCGCTTACTGG911000.14788805468410007No Hit
CGTGGTCGTATTTAGCCTTAGATGGAGTTTACCACCCACTTAGGGCTGCA905150.14693838934941073No Hit
GTGATATCGTCGCAACCTTGCCTAGACGGGATTCGGCCTTAGAGGCGTTC872120.14157643276739557No Hit
CGCAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTG848840.13779725174319596No Hit
GTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAAATTGGCCCACTT846920.13748556671027226No Hit
CTCCACATCAGCCCTCCAGGCCCTCGCTAGAGTATTTGCTACTACCACCA825370.13398722688761325No Hit
CTTTTCTGACACCTCTTGCTGAAATCTCTTCAAGCCAAAAGGATCGACAG823280.13364794474239947No Hit
CCTGGCTGTGTCCTCGAATCGGATCACGCGAGGGAGTGTTTGCCGGTCGG821890.1334222977654391No Hit
CCGCCGTTTACCCGCGCTTGCTTGAATTTCTTCACTTTGACATTCAGAGC806850.13098076500753691No Hit
GTCGCGACGTCCTACAGGAAGAGAAGTGCGGTCCTCCGCCGCCGGACGAC798720.12966097369625074No Hit
CGCAGACAGGTTCACGCGGGAACGGACGGAGACCTTACCGCCAACAGCGC745500.12102145418989747No Hit
GTCAAACTCCCCGCCTGGCTGTGTCCTCGAATCGGATCACGCGAGGGAGT740800.12025847520305305No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA723520.11745330990673994No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCGAGCTTATGACTCGCGCT714920.11605722069676928No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC707470.11484781783464355No Hit
GTCACTACCTCCCCGTGCCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT702090.11397445039863867No Hit
CCAGGCCCTCGCTAGAGTATTTGCTACTACCACCAAGATCTGCACCGACG697060.11315790054676049No Hit
CGGGCGTTTGGTTCATCCCACAGCGCCAGTTCTGCTTACCAAAATTGGCC697010.1131497837490281No Hit
GGCATAGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCT694760.11278452785107067No Hit
CAGAAATCACATTGCGTAAACACCCAGCTGGGGCCATCGCAATGCTTTGC692740.11245660922268222No Hit
ATTTAGCCTTAGATGGAGTTTACCACCCACTTAGGGCTGCACTCTCAAGC692620.11243712890812448No Hit
CTCCTGGTGATATCGTCGCAACCTTGCCTAGACGGGATTCGGCCTTAGAG687600.11162220241579277No Hit
GTCGATTCGGGCGCCTTAACCGGGCGTTTGGTTCATCCCACAGCGCCAGT687270.11156863155075902No Hit
AGAAATTTGACCGCCGGTAGCACGATTGTGGATACCGGGTACGCCTAGTT685200.11123259612463816No Hit
GCCGTGCTTTCGCAGTCCCTATGCGTACTGGACATCGGGATCAAACCAGC684630.11114006463048896No Hit
GCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATCGACGAG681150.11057513550831478No Hit
CACAGATCCAACTACGAGCTTTTTAACCGCAACAACTTTAATATACGCTA673580.10934625233163131No Hit
ATTGATCATTACCTCAGATTCCGAAAACCAACAAAATAGAACCGAGGTCC669110.10862061061435588No Hit
CGTCGATTGGTTTTGATCTAATAAAAGCGTCCCTTCCATTTCTGGTCGGG666520.10820016049181819No Hit
TCAACATCGGATTTCTCCTAGGGCTTAGGGTCGACTGACTCTTGTGCAAC664990.10795178648120715No Hit
AGCGCGCGTGCGGCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGC663180.10765795840329473No Hit
GCTCAGTCTCTTGCGGCTAGAAGCCGCCTGTCCCTCTAAGAAGAAATTTG659550.10706867888792337No Hit
TGTCCCTCTAAGAAGAAATTTGACCGCCGGTAGCACGATTGTGGATACCG653600.10610277995776926No Hit
CTCGAATCGGATCACGCGAGGGAGTGTTTGCCGGTCGGACACGCCCGGTA648920.10534304769001782No Hit
CCCCGTTACCCGTTACAACCATGGTAGGCGCAGAACCTACCATCGACAGT648020.10519694533083483No Hit
CGCTTATAAAGAAAAGACAACTCTTCCCAGACCTCCCGCCGGCGTCTCCG644320.10459630229863814No Hit
CCCTGTGGTAACTTTTCTGACACCTCTTGCTGAAATCTCTTCAAGCCAAA640490.10397455559233727No Hit
CGGGTGAACAATCCGACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAG640430.10396481543505842No Hit
CCCCGCTCGGTACCTAAGTCGTCTACAGACGATTCCGAGTCCCGACATCG630350.1023284690122091No Hit
CCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAGTCTCTTGCG629160.10213528922617827No Hit
AAGAAATTTGACCGCCGGTAGCACGATTGTGGATACCGGGTACGCCTAGT628530.1020330175747502No Hit
TGATCATTACCTCAGATTCCGAAAACCAACAAAATAGAACCGAGGTCCTA625330.10151354251987739No Hit
GTAACTTTTCTGACACCTCTTGCTGAAATCTCTTCAAGCCAAAAGGATCG622770.10109796247597914No Hit
CTCGGTACCTAAGTCGTCTACAGACGATTCCGAGTCCCGACATCGAACTC618420.10039180107326144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)