FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_hy_mut_bra1.351000000b882d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_hy_mut_bra1.351000000b882d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7486796
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT344070.4595690866960981TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA56550.031.1183236-37
GTATGCC60050.028.83349446-47
TATGCCG63700.027.66494648-49
GTGGCCT64800.027.33900834-35
GCCTTAT66050.026.10323736-37
ATGCCGT66450.026.09115248-49
TGGCCTT69100.025.0878734-35
CGTATGC72400.024.66959646-47
TGCTTGA73400.024.33122360-61
GCCGTCT71750.024.3283150-51
CGTGGCC73500.023.74771532-33
CCGTCTT75450.023.70208752-53
TGCCGTC76200.023.37506350-51
CCTTATC77400.022.79764638-39
CTGCTTG75950.022.76415658-59
TCGTATG77900.022.34780144-45
GCTTGAA79250.021.99575860-61
CCAGTCA80300.021.91399626-27
GGTCATA26300.021.8509254
CTCTTAT69850.021.350568
Produced by FastQC (version 0.11.2)