FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_hy_con_bra4.351000000b8860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_hy_con_bra4.351000000b8860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5502486
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT335880.6104150015102265TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC51900.031.62285246-47
TATGCCG54000.030.6120248-49
ATGCCGT58150.028.18226848-49
GCCGTCT59300.027.59417550-51
ACCGTAC60300.027.5681732-33
AGTCACC62250.026.5517528-29
TGCTTGA62450.026.50643260-61
CCGTCTT63300.026.3380952-53
CTGCTTG62900.026.16624558-59
CACCGTA62600.025.9862430-31
CCGTACG62800.025.86566732-33
CGTATGC64800.025.80405846-47
CCAGTCA64800.025.4700126-27
GCTTGAA65750.025.03158860-61
TGCCGTC66850.025.01060150-51
TCGTATG66450.024.94773344-45
GTAATCT68150.024.32342138-39
CAGTCAC67350.023.9767726-27
CTCGTAT72250.023.17511644-45
CTCCAGT71600.023.15036224-25
Produced by FastQC (version 0.11.2)