FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_hy_con_bra2.351000000b883a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_hy_con_bra2.351000000b883a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4070594
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT193620.475655395748139TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC34050.029.22706646-47
TATGCCG36650.027.47667548-49
ATGCCGT39200.025.3863548-49
CGTATGC40850.024.7107146-47
TGCTTGA42450.024.4477460-61
GCCGTCT41250.024.29626350-51
CCGTCTT44250.023.18548252-53
TCGTATG43100.023.08920944-45
TGCCGTC44550.022.92301650-51
CCAGTCA44500.022.84048826-27
GCTTGAA44700.022.7921260-61
CTGCTTG44700.022.57960758-59
GGAATCT45550.022.00170738-39
CAGTCAC46650.021.63510126-27
AGTCACG48100.021.27899728-29
GTTTCGG49100.020.94234834-35
CTCGTAT49500.020.82364844-45
TCCAGTC48750.020.70325324-25
CGGAATC49800.020.69629938-39
ACTCCAG49450.020.26611322-23
Produced by FastQC (version 0.11.2)