FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g5_ov.351000000b8798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g5_ov.351000000b8798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312680
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT43660.13179661180675464TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT9850.014.5410841
GTATGCC15900.014.34037746-47
TATGCCG17900.013.135571548-49
CGGGCAT8850.012.40784551
CCCCACT5958.212737E-912.0361081
CGTATGC20100.011.69837846-47
GCCGTCT21150.011.67813250-51
CTCTTAT21650.011.407318
CCAGTCA20300.011.34714926-27
CTCAGAT14350.011.3119841
CTAGACT12700.011.219038
TCACATG21850.011.19455130-31
TCTAGAC13500.010.9060057
AGTCACA21800.010.89309428-29
CGGGTAT9355.456968E-1210.7230781
ATGCCGT20600.010.72216548-49
CTCATTT23350.010.6323251
TCTCTTA26500.010.5741137
CAGTCAC21400.010.54194826-27
CGGGAAT15500.010.4727091
Produced by FastQC (version 0.11.2)