FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g5_fb.351000000b877e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g5_fb.351000000b877e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103433
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT38240.1232183842860471TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATTT19500.017.6251051
TCTAGAC16950.015.9714127
CTAGACT17350.015.3294548
TAAACCT14250.014.6647948
CTGGAAT16000.014.3203971
CGTATGC16300.013.5519446-47
CCGTATC17250.012.3907238-39
TTAAACC17150.012.1850327
TAGACTT23000.012.1832279
GTATGCC17200.012.014256546-47
TTCCGTA18350.011.90660436-37
CATTCTA24350.011.8974814
TATGCCG19450.011.60101848-49
GTCACAG18900.011.55974228-29
CGGGGTT5807.458402E-811.522161
ATTCTAG25250.011.4737835
ATGCCGT17750.011.37398748-49
CTAGGAT7158.931238E-1011.34951
TTCTAGA26100.011.28208356
CCGTCTT19850.011.12685952-53
Produced by FastQC (version 0.11.2)