FastQCFastQC Report
Thu 11 Jan 2018
H57W7BCX2_l02n01_d3_g5_br.351000000b8755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH57W7BCX2_l02n01_d3_g5_br.351000000b8755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3042227
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT52190.1715519584830455TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT20450.018.1147468
GTATGCC17200.018.08991846-47
TATGCCG19550.016.15734748-49
TCTCTTA25800.014.910647
AGTCAAC22250.014.08831834-35
CGTATGC22350.013.81527746-47
GTCACAG22450.013.53947228-29
CCAGTCA23250.013.48214626-27
TCGTATG22750.013.46708244-45
CCGGGTT6409.094947E-1213.4234121
ATGCCGT23150.013.33698248-49
GCCGTCT23450.013.26687650-51
CCGTCTT24100.013.10646352-53
CACAGTC24100.013.00663730-31
TCACAGT23850.012.94383730-31
CTGGGAT10350.012.91183951
TGCTTGA24200.012.85560760-61
CAGTCAC24700.012.4022626-27
CTGCTTG26100.012.1929358-59
ACTCCAG24550.012.1878522-23
Produced by FastQC (version 0.11.2)